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Phosphorylation of B-50 (GAP43) Is Correlated with Neurotransmitter Release in Rat Hippocampal Slices

Authors

  • L. V. Dekker,

    1. Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Utrecht, The Netherlands
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  • P. N. E. De Graan,

    Corresponding author
    1. Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Utrecht, The Netherlands
      Address correspondence and reprint requests to P. N. E. De Graan at Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.
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  • D. H. G. Versteeg,

    1. Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Utrecht, The Netherlands
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  • A. B. Oestreicher,

    1. Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Utrecht, The Netherlands
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  • W. H. Gispen

    1. Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Utrecht, The Netherlands
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Address correspondence and reprint requests to P. N. E. De Graan at Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, and Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Abstract

Abstract: Recent studies have demonstrated that phorbol diesters enhance the release of various neurotransmitters. It is generally accepted that activation of protein kinase C (PKC) is the mechanism by which phorbol diesters act on neurotransmitter release. The action of PKC in neurotransmitter release is very likely mediated by phosphorylation of substrate proteins localized in the presynaptic nerve terminal. An important presynaptic substrate of PKC is B-50. To investigate whether B-50 mediates the actions of PKC in neurotransmitter release, we have studied B-50 phosphorylation in intact rat hippocampal slices under conditions that stimulate or inhibit PKC and neurotransmitter release. The slices were labelled with [32P]orthophosphate. After treatment, the slices were homogenized, B-50 was immunoprecipitated from the slice homogenate, and the incorporation of 32P into B-50 was determined. Chemical depolarization (30 μM K+) and the presence of phorbol diesters, conditions that stimulate neurotransmitter release, separately and in combination, also enhance B-50 phosphorylation. Polymyxin B, an inhibitor of PKC and neurotransmitter release, decreases concentration dependently the depolarization-induced stimulation of B-50 phosphorylation. The effects of depolarization are not detectable at low extracellular Ca2+ concentrations. It is concluded that in rat hippocampal slices B-50 may mediate the action of PKC in neurotransmitter release.

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