N-Cadherin Gene Maps to Human Chromosome 18 and Is Not Linked to the E-Cadherin Gene
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 55, Issue 3, pages 805–812, September 1990
How to Cite
Walsh, F. S., Barton, C. H., Putt, W., Moore, S. E., Kelsell, D., Spurr, N. and Goodfellow, P. N. (1990), N-Cadherin Gene Maps to Human Chromosome 18 and Is Not Linked to the E-Cadherin Gene. Journal of Neurochemistry, 55: 805–812. doi: 10.1111/j.1471-4159.1990.tb04563.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received November 23, 1989; revised manuscript received January 6, 1990; accepted January 29, 1990.
- Cell adhesion molecules;
- Gene mapping
Abstract: cDNA clones encoding the human N-cadherin cell adhesion molecule have been isolated from an embryonic muscle library by screening with an oligonucleotide probe complementary to the chick brain sequence and chick brain cDNA probe λN2. Comparison of the predicted protein sequences revealed >91% homology between chick brain, mouse brain, and human muscle N-cadherin cDNAs over the 748 amino acids of the mature, processed protein. A single polyadenylation site in the chick clone was also present and duplicated in the human muscle sequence. Immediately 3’of the recognition site in chick a poly(A) tail ensued; however, in human an additional 800 bp of 3’untranslated sequence followed. Northern analysis identified a number of major N-cadherin mRNAs. These were of 5.2, 4.3, and 4.0 kb in C6 glioma, 4.3 and 4.0 kb in human foetal muscle cultures, and 4.3 kb in human embryonic brain and mouse brain with minor bands of 5.2 kb in human muscle and embryonic brain. Southern analysis of a panel of somatic cell hybrids allowed the human N-cadherin gene to be mapped to chromosome 18. This is distinct from the E-cadherin locus on chromosome 16. Therefore, it is likely that the cadherins have evolved from a common precursor gene that has undergone duplication and migration to other chromosomal locations.