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Identification of Meningeal Cell Released Neurite Promoting Activities for Embryonic Hippocampal Neurons

Authors

  • H. Peter Matthiessen,

    Corresponding author
    1. Molecular Neurobiology Laboratory, Department of Neurology, University of Düsseldorf, Düsseldorf, F.R.G.
      Address correspondence and reprint requests to Dr. H. P. Matthiessen at Molecular Neurobiology Laboratory, Department of Neurology, University of Düsseldorf, Moorenstr. 5, D-4000 Düsseldorf 1, F.R.G.
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  • Corinne Schmalenbach,

    1. Molecular Neurobiology Laboratory, Department of Neurology, University of Düsseldorf, Düsseldorf, F.R.G.
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  • Hans Werner Müller

    1. Molecular Neurobiology Laboratory, Department of Neurology, University of Düsseldorf, Düsseldorf, F.R.G.
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Address correspondence and reprint requests to Dr. H. P. Matthiessen at Molecular Neurobiology Laboratory, Department of Neurology, University of Düsseldorf, Moorenstr. 5, D-4000 Düsseldorf 1, F.R.G.

Abstract

Abstract: Primary cultures of meningeal cells from embryonic rat cerebra secrete neurite growth-inducing components into serum-free culture medium. This conditioned medium (CM) was analyzed by FPLC and immunochemical and enzymatic treatments and tested for neurite promoting activity (NPA) in a quantitative bioassay using hippocampal neurons from embryonic rat. By immunoprecipitation or specific adsorption we identified laminin (LN)-proteoglycan complexes and fibronectin (FN), respectively, as the major neurite promoting components within meningeal cell CM. The LN-proteoglycan complexes and their NPA were sensitive to chondroitinase (chondroitin ABC lyase, EC 4.2.2.4) and to a smaller extent to heparitinase (heparitin sulfate lyase, EC 4.2.2.8). Minor fractions of the total NPA in CM correlated with free LN and a putative but not yet characterized FN-proteoglycan complex.

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