Distinct, Developmentally Regulated Brain mRNAs Direct the Synthesis of Neurotransmitter Transporters

Authors

  • Randy D. Blakely,

    Corresponding author
    1. Section of Molecular Neurobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
      Address correspondence and reprint requests to Dr. R. D. Blakely at his present address: Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, U.S.A.
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  • Janet A. Clark,

    1. Section of Molecular Neurobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
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  • Tadeusz Pacholczyk,

    1. Section of Molecular Neurobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
    2. Program in Neuroscience, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
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  • Susan G. Amara

    1. Section of Molecular Neurobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
    2. Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
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Address correspondence and reprint requests to Dr. R. D. Blakely at his present address: Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, U.S.A.

Abstract

Abstract: The Xenopus laevis oocyte expression system was utilized to define developmental and structural properties of neurotransmitter transporter mRNAs and the pharmacological characteristics of encoded carriers independent of the complexities of brain tissue preparations. Poly(A)+ RNA from dissected brain regions of neonatal and adult rats was microinjected into Xenopus oocytes and the expression of Na+-dependent neurotransmitter transporters determined 48 h later. Transport studies conducted with oocytes injected with RNAs derived from juvenile rat tissues indicate a region- and transporter-specific, postnatal increase in mRNA abundance as a major factor in the developmental changes observed for brain high-affinity amino acid uptake systems. Both L-glutamic acid (Glu) and γ-aminobutyric acid (GABA) uptake systems were detectable by day 3 in postnatal forebrain mRNA and became progressively enriched during the next 2 weeks of forebrain development. In contrast, brainstem Glu and GABA transporter enrichment was 60–70% of adult values by day 3 and exceeded adult levels by day 10. Parallel determinations of L-glutamic acid decarboxylase mRNA abundance during development argue for distinct regulatory influences on mRNAs directing transmitter synthesis and reuptake. Glycine uptake could not be detected at any point of forebrain development and exhibited a gradual postnatal rise to adult levels over the first 3 postnatal weeks of brainstem development. Uptake studies conducted with well-characterized inhibitors of Glu, GABA, dopamine, and choline transport (D-aspartate, nipecotic acid, nomifensine, and hemicholinium-3, respectively) revealed that oocyte transporters encoded by adult rat brain mRNAs retained antagonist sensitivities exhibited by in vitro brain preparations. In addition, a differential regional sensitivity to the Glu transport antagonist dihydrokainate (1 mM) was observed, lending support to previous reports of region-specific Glu transporter subtypes. To determine the structural diversity present among brain transporter mRNAs, poly(A)+ RNA was size-fractionated on linear (10–31%) sucrose density gradients prior to oocyte injection. These experiments revealed two mRNA size classes (2.4–3.0 kb, 4.0–4.5 kb) independently capable of directing the synthesis of Glu, GABA, and glycine transporters. In regions other than the cerebellum, Glu and GABA transporter activities migrated as single, yet distinct, peaks of 4.0–4.5 kb. In contrast, both Glu and GABA transporters exhibited major peaks of activity at 2.5–3.0 kb with size-fractionated cerebellar mRNA. Brainstem glycine uptake exhibited a broad sedimentation profile, with peaks apparent at 2.4 and 4.0 kb. Taken together, these findings indicate previously unappreciated complexity in mRNA structure and regulation which underlies the expression of amino acid neurotransmitter uptake systems in the rodent CNS.

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