The present address of Dr. A. Wieraszko is Department of Biology, CSI/IBR Center for Developmental Neuroscience, The City University of New York at the College of Staten Island, Staten Island, NY, 10301, U.S.A.
Enhanced Aspartate Release from Hippocampal Slices of Epileptic (El) Mice
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 56, Issue 3, pages 1007–1011, March 1991
How to Cite
Flavin, H. J., Wieraszko, A. and Seyfried, T. N. (1991), Enhanced Aspartate Release from Hippocampal Slices of Epileptic (El) Mice. Journal of Neurochemistry, 56: 1007–1011. doi: 10.1111/j.1471-4159.1991.tb02021.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Recieved April 18, 1990; revised August 22, 1990; accepted September 9, 1990
- Epileptic (El) mice;
- Hippocampal slices;
- Potassium depolarization;
- Exicitatory amino acid release
Abstract: The release of putative neurotransmitters [aspartate, glutamate, and γ-aminobutyric acid (GABA)] was studied in hippocampal slices from adult normal C57BL/6J (B6) and El (epileptic) mice. The El mice, a genetic model of temporal lobe epilepsy, had an average of 86 seizures. Sets of B6 and El hippocampal slices (400 μm thick) were incubated in a series of normal and high potassium (60 mM) buffers in the presence or absence of calcium. The calcium-dependent and calcium-independent potassium-induced release of amino acids was compared in each mouse strain. Release of endogenous amino acids was measured using liquid chromatog-raphy with electrochemical detection and was expressed as picomoles of amino acid released per milliliter of incubation buffer per minute of incubation per slice ± SEM. No significant differences were found between the El and B6 mice for the calcium-dependent potassium-evoked release of glutamate (18.20 ± 2.62 and 15.41 ± 3.56), or GABA (17.28 ± 2.90 and 12.73 ± 1.37), respectively. Aspartate release, however, was significantly higher in the El mice (6.62 ± 0.69) than in the B6 mice (3.31 ± 0.72). These findings suggest that enhanced aspartate release may be related to seizure expression in El mice.