One-Line Monitoring of Extracellular Brain Glucose Using Microdialysis and a NADPH-Linked Enzymatic Assay
Version of Record online: 5 OCT 2006
Journal of Neurochemistry
Volume 57, Issue 2, pages 648–654, August 1991
How to Cite
van der Kuil, J. H. F. and Korf, J. (1991), One-Line Monitoring of Extracellular Brain Glucose Using Microdialysis and a NADPH-Linked Enzymatic Assay. Journal of Neurochemistry, 57: 648–654. doi: 10.1111/j.1471-4159.1991.tb03796.x
- Issue online: 5 OCT 2006
- Version of Record online: 5 OCT 2006
- Received August 27, 1990; revised manuscript received January 3, 1991; accepted January 22, 1991.
- On-line monitoring;
- Extracellular space
A method to monitor extracellular glucose in freely moving rats, based on intracerebral microdialysis coupled to an enzyme reactor is described. The dialysate is continuously mixed with a solution containing the enzymes hexokinase and glucose-6-phosphate dehydrogenase, and the fluorescence of NADPH formed enables the on-line registration of extracellular glucose. The method is applied to monitor changes in extracellular brian glucose during the infusion of glucose, electrically induced seizure, immobilization stress, and repetitive hypoxia. After glucose loading or after seizure, hippocampus dialysate glucose concentration was increased transiently. During immobilization, there was a short-lasting decrease and, therafter, an increase in the extracellular hippocampus glucose. During repetitive hypoxia in rats with a unilaterally occluded carotid artery, the content of glucose of striatal dialysates followed closely changfes in blood pressure. These results illustrate the usefulness of the method in studying changes in brain glucose concentrations underpathological and physiological conditions.