Pre-and Postnatal Developmental Changes of Adrenoceptor Subtypes in Rat Brain
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 57, Issue 3, pages 897–903, September 1991
How to Cite
Erdtsieck-Ernste, B. H. W., Feenstra, M. G. P. and Boer, G. J. (1991), Pre-and Postnatal Developmental Changes of Adrenoceptor Subtypes in Rat Brain. Journal of Neurochemistry, 57: 897–903. doi: 10.1111/j.1471-4159.1991.tb08235.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received November 7, 1990; revised manuscript received January 10, 1991; accepted February 6, 1991.
- β-Adrenergic receptors;
- Brain development;
- β1 and β2 subtypes;
β-Adrenergic receptor subtypes, β1 and β2, were studied during pre-and postnatal development in the rat brain. [125I]Iodocyanopindolol (6–300 pmol/L) binding assays in the presence of 5-hydroxytryptamine (0.6–6 μmol/L) were used to measure exclusively β-adrenergic receptors. In forebrain tissue, saturable and stereoselective binding was detected on gestational day 13. The amount of β-adrenergic binding increased until postnatal day 23, when adult values were reached. The dissociation constants of [125I]iodocyanopindolol binding remained the same throughout development, as did the affinity of several β-adrenergic and non-β-adrenergic compounds. The proportion of the β2-adrenergic receptors was determined using the β1-selective antagonist ICI-89406 (7–150 nmol/L) and was found to change from 65% in prenatal forebrain tissue to 28% in adulthood. In cerebellum/medulla pons tissue, however, the proportion of β2-receptor binding (80%) remained unchanged during the whole developmental period.