Present address of F. Martín is Department of Physiology, Faculty of Biological Sciences, University of Santiago, Santiago de Compostela, Spain.
Simultaneous Effects of p-Chloroamphetamine, d-Fenfluramine, and Reserpine on Free and Stored 5-Hydroxytryptamine in Brain and Blood
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 59, Issue 3, pages 1138–1144, September 1992
How to Cite
Martín, F. and Artigas, F. (1992), Simultaneous Effects of p-Chloroamphetamine, d-Fenfluramine, and Reserpine on Free and Stored 5-Hydroxytryptamine in Brain and Blood. Journal of Neurochemistry, 59: 1138–1144. doi: 10.1111/j.1471-4159.1992.tb08356.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received January 20, 1992; revised manuscript received February 14, 1992; accepted March 12, 1992
- Serotonin release;
- Cerebrospinal fluid;
- 5-Hydroxyindoleacetic acid
Abstract: The effects of acute treatment with p-chloroamphetamine, d-fenfluramine, and reserpine on intracellular (brain tissue and whole blood) and extracellular (CSF and platelet-free plasma) compartments of 5-hydroxytryptamine (5-HT) in the brain and blood of the same rats have been examined. These treatments affected 5-HT in brain tissue and whole blood similarly (r= 0.823). Reserpine significantly reduced both intracellular pools at 2 and 24 h. p-Chloroamphetamine and d-fenfluramine were more effective on brain tissue 5-HT. The concentration of 5-HT in CSF was significantly increased by all treatments. p-Chloroamphetamine induced a dramatic 70-fold increase of CSF 5-HT, paralleling a 42% decrease in brain tissue. d-Fenfluramine significantly increased CSF 5-HT to 212% of controls and reduced whole brain 5-HT (-23%). The effects of p-chloroamphetamine and d-fenfluramine on 5-HIAA in brain, CSF, and plasma were nonsignificant. Individual values of 5-hydroxyindoleacetic acid (5-HIAA) in CSF and brain were highly correlated (r= 0.855), indicating that CSF 5-HIAA reflects well the concentration of 5-HIAA in brain tissue. Yet the intra- and extracellular concentrations of 5-HIAA were unrelated to the 5-HT changes. This indicates that CSF 5-HIAA does not reflect the active (extracellular) compartment of 5-HT in brain.