Human Brain Lectin: A Soluble Lectin That Binds Actin

Authors


Address correspondence and reprint requests to Dr. M. Caron at Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, 74, rue Marcel Cachin, 93012 Bobigny Cedex, France.

Abstract

Abstract: A biotinylated probe was used for detection of endogenous ligands of a human brain lectin on blotted human brain soluble proteins. Of the various proteins from brain extract resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, five reacted with the biotinylated probe. After elimination of saccharidic moieties by periodic treatment of the same extract, a single band with Mr∼ 43,000 was recognized by the lectin. This band was identified as actin using an anti-actin antibody. These results were confirmed by binding of biotinylated lectin to purified actin.

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