Secretogranin II Is Synthesized and Secreted in Astrocyte Cultures
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 60, Issue 6, pages 2312–2314, June 1993
How to Cite
Fischer-Colbrie, R., Kirchmair, R., Schobert, A., Olenik, C., Meyer, D. K. and Winkler, H. (1993), Secretogranin II Is Synthesized and Secreted in Astrocyte Cultures. Journal of Neurochemistry, 60: 2312–2314. doi: 10.1111/j.1471-4159.1993.tb03520.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Resubmitted manuscript received February 5, 1993; accepted February 17, 1993.
- Carboxypeptidase H;
Abstract: Astrocyte cultures from rat brain were analyzed for their ability to synthesize and secrete secretogranin II (chromogranin C). Northern blot analysis of polyA-selected RNA established the presence of secretogranin II mRNA in these cells. By radioimmunoassay, 11.6 fmol/106 astrocytes of secretogranin II was found in these cells. About twice the amount was released into the medium within 3 days. Secretogranin II within the astrocytes was practically unprocessed, as shown by HPLC. These results establish for the first time that astrocytes in vitro synthesize and sec rete a protein of the acidic chromogranin family.