Drosophilaγ-Aminobutyric Acid Receptor Gene Rdl Shows Extensive Alternative Splicing
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 60, Issue 6, pages 2323–2326, June 1993
How to Cite
Ffrench-Constant, R. H. and Rocheleau, T. A. (1993), Drosophilaγ-Aminobutyric Acid Receptor Gene Rdl Shows Extensive Alternative Splicing. Journal of Neurochemistry, 60: 2323–2326. doi: 10.1111/j.1471-4159.1993.tb03523.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Resubmitted manuscript received February 24, 1993; accepted March 1, 1993.
- γ-Aminobutyric acid receptor;
- Alternative splicing;
- Drosophila melanogaster;
- Insecticide resistance.
Abstract: The Drosophilaγ-aminobutyric acid (GABA) receptor subunit gene Rdl was isolated on the basis of a mutant phenotype showing high levels of insensitivity to picrotoxinin and cyclodiene insecticides. Following analysis of two dissimilar cDNAs isolated from the locus, we report that Rdl undergoes extensive alternative splicing at two locations in the putative extracellular domain. At each location a choice is made between exons of the same size: “a'’or “b'’(23 amino acids long with two substitutions) and “c'’or “D'’(46 residues long with 10 substitutions). The function of these alternative exons remains unclear; however, exon d contains a putative site for casein kinase II phosphorylation. AH possible combinations of exons (a with c or d and b with c or d) were found in RNA isolated from early embryos. This is the first demonstration of alternative splicing in a GABA receptor gene from invertebrates.