Ca2+/Calmodulin-Dependent Protein Kinase II and Protein Kinase C Phosphorylate a Synthetic Peptide Corresponding to a Sequence That Is Specific for the γ2L Subunit of the GABAA Receptor

Authors

  • Tina K. Machu,

    Corresponding author
    1. Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado, U.S.A.
      Address correspondence and reprint requests to Dr. T. K. Machu at Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C-236, Denver, CO 80262, U.S.A.
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  • Jordan A. Firestone,

    1. Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado, U.S.A.
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  • Michael D. Browning

    1. Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado, U.S.A.
    2. Program in Neurosciences, University of Colorado Health Sciences Center, Denver, Colorado, U.S.A.
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Address correspondence and reprint requests to Dr. T. K. Machu at Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C-236, Denver, CO 80262, U.S.A.

Abstract

Abstract: The γ2 subunit of the GABAA receptor (GABAA-R) is alternatively spliced. The long variant (γ2L) contains eight additional amino acids that possess a consensus sequence site for protein phosphorylation. Previous studies have demonstrated that a peptide or fusion protein containing these eight amino acids is a substrate for protein kinase C (PKC), but not cyclic AMP-dependent protein kinase A (PKA)-stimulated phosphorylation. We have examined the ability of PKA, PKC, and Ca2+/calmodulin-dependent protein kinase (CAM kinase II) to phosphorylate a synthetic peptide corresponding to residues 336–351 of the intracellular loop of the γ2L subunit and inclusive of the alternatively spliced phosphorylation consensus sequence site. PKC and CAM kinase II produced significant phosphorylation of this peptide, but PKA was ineffective. The Km values for PKC-and CAM kinase II-stimulated phosphorylation of this peptide were 102 and 35 μM, respectively. Maximal velocities of 678 and 278 nmol of phosphate/min/mg were achieved by PKC and CAM kinase II, respectively. The phosphorylation site in the eight-amino-acid insert of the γ2L subunit has been shown to be necessary for ethanol potentiation of the GABAA-R. Thus, our results suggest that PKC, CAM kinase II, or both may play a role in the effects of ethanol on GABAergic function.

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