Characterization of the Scavenger Receptor on Bovine Cerebral Endothelial Cells In Vitro
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 61, Issue 5, pages 1813–1821, November 1993
How to Cite
de Vries, H. E., Kuiper, J., de Boer, A. G., van Berkel, Th. J. C. and Breimer, D. D. (1993), Characterization of the Scavenger Receptor on Bovine Cerebral Endothelial Cells In Vitro. Journal of Neurochemistry, 61: 1813–1821. doi: 10.1111/j.1471-4159.1993.tb09821.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Recieved November 30, 1992; revised March 19, 1993; accepted March 19, 1993.
- Blood-brain barrie;
- erebral endothelial cell;
- cetylated low density lipoprotei;
- cavenger receptor
Abstract— Primary cultures of bovine brain capillary endo-thelial cells (BCEC), possessing tight junctions and high levels of γ-glutamyl transpeptidase, were used as an in vitro model for the blood-brain barrier. The interaction of acetylated low density lipoprotein (AcLDL) with BCEC was studied to characterize the scavenger receptor on these cells. A saturable high affinity binding site was found with a dissociation constant of AcLDL of 5.4 μg/ml (3.1 nM) and a maximal binding ranging from 284 to 626 ng of AcLDL/mg of cell protein for eight primary cultures, and independent of the presence of calcium. Cell association was coupled to degradation, and both could be effectively competed for by polyinosinic acid and AcLDL but not by low density lipoprotein or by high density lipoprotein. Prolonged incubation showed an accumulation of the ligand in the cells. The rate of degradation of AcLDL was ∼ 10–20-fold lower in BCEC than that of peripheral endothelial cells. No evidence for lysosomal degradation could be obtained. Binding of 1,1′-dioctadecyl-3,3,3,3′-tetramethylindocar-boxyamine perchlorate-labeled AcLDL by BCEC was observed, which could be competed for by an excess of un-labeled AcLDL and polyinosinic acid. We have shown that in vitro BCEC possesses specific binding sites for AcLDL, whereas these cells show a relatively low degradative capacity.