Docosahexaenoic acid promotes neurite growth in hippocampal neurons
Article first published online: 13 JUL 2004
Journal of Neurochemistry
Volume 90, Issue 4, pages 979–988, August 2004
How to Cite
Calderon, F. and Kim, H.-Y. (2004), Docosahexaenoic acid promotes neurite growth in hippocampal neurons. Journal of Neurochemistry, 90: 979–988. doi: 10.1111/j.1471-4159.2004.02520.x
- Issue published online: 13 JUL 2004
- Article first published online: 13 JUL 2004
- Received December 23, 2003; revised manuscript received March 12, 2004; accepted 12 March, 2004.
- docosahexaenoic acid;
- n-3 fatty acid deficiency;
- neurite growth;
- primary culture
Docosahexanoic acid (22:6n-3; DHA) deficiency during development is associated with impairment in learning and memory, suggesting an important role of DHA in neuronal development. Here we provide evidence that DHA promotes neuronal differentiation in rat embryonic hippocampal primary cultures. DHA deficiency in vitro was spontaneously induced by culturing hippocampal cells in chemically defined medium. DHA supplementation improved DHA levels to values observed in freshly isolated hippocampus. We found that DHA supplementation in culture increased the population of neurons with longer neurite length per neuron and with higher number of branches. However, supplementation with arachidonic, oleic or docosapentaenoic acid did not have any effect, indicating specificity of the DHA action on neurite growth. Furthermore, hippocampal cultures obtained from n-3 fatty acid deficient animals contained a lower DHA level and a neuronal population with shorter neurite length per neuron in comparison to those obtained from animals with adequate n-3 fatty acids. DHA supplementation to the deficient group recovered the neurite length to the level similar to n-3 fatty acid adequate cultures. Our data demonstrates that DHA uniquely promotes neurite growth in hippocampal neurons. Inadequate neurite development due to DHA deficiency may contribute to the cognitive impairment associated with n-3 fatty acid deficiency.