Localization of organic anion transporting polypeptide 3 (oatp3) in mouse brain parenchymal and capillary endothelial cells
Article first published online: 2 JUL 2004
Journal of Neurochemistry
Volume 90, Issue 3, pages 743–749, August 2004
How to Cite
Ohtsuki, S., Takizawa, T., Takanaga, H., Hori, S., Hosoya, K.-i. and Terasaki, T. (2004), Localization of organic anion transporting polypeptide 3 (oatp3) in mouse brain parenchymal and capillary endothelial cells. Journal of Neurochemistry, 90: 743–749. doi: 10.1111/j.1471-4159.2004.02549.x
- Issue published online: 2 JUL 2004
- Article first published online: 2 JUL 2004
- Received January 13, 2004; revised manuscript received March 19, 2004; accepted April 8, 2004.
- blood–brain barrier;
- choroid plexus;
- olfactory bulb;
- organic anion transporting polypeptide 3
Organic anion transporting polypeptide 3 (oatp3) transports various CNS-acting endogenous compounds, including thyroid hormones and prostaglandin E2, between extra- and intracellular spaces, suggesting a possible role in CNS function. The purpose of this study was to clarify the expression and localization of oatp3 in the mouse brain. RT-PCR analysis revealed that oatp3 mRNA is expressed in brain capillary-rich fraction, conditionally immortalized brain capillary endothelial cells, choroid plexus, brain and lung, but not in liver or kidney, where oatp1, 2 and 5 mRNAs were detected. Immunohistochemical analysis with anti-oatp3 antibody suggests that oatp3 protein is localized at the brush-border membrane of mouse choroid plexus epithelial cells. Furthermore, intense immunoreactivity was detected in neural cells in the border region between hypothalamus and thalamus, and in the olfactory bulb. Immunoreactivity was also detected in brain capillary endothelial cells in the cerebral cortex. These localizations in the mouse brain suggest that oatp3 plays roles in blood–brainand –cerebrospinal fluid barrier transport of organic anions and signal mediators, and in hormone uptake by neural cells.