Gene expression profiling of individual cases reveals consistent transcriptional changes in alcoholic human brain
Article first published online: 13 JUL 2004
Journal of Neurochemistry
Volume 90, Issue 5, pages 1050–1058, September 2004
How to Cite
Liu, J., Lewohl, J. M., Dodd, P. R., Randall, P. K., Harris, R. A. and Mayfield, R. D. (2004), Gene expression profiling of individual cases reveals consistent transcriptional changes in alcoholic human brain. Journal of Neurochemistry, 90: 1050–1058. doi: 10.1111/j.1471-4159.2004.02570.x
- Issue published online: 27 JUL 2004
- Article first published online: 13 JUL 2004
- Received February 5, 2004; revised manuscript received April 8, 2004; accepted April 13, 2004.
- frontal cortex;
- gene expression;
- motor cortex
Chronic alcohol exposure induces lasting behavioral changes, tolerance, and dependence. This results, at least partially, from neural adaptations at a cellular level. Previous genome-wide gene expression studies using pooled human brain samples showed that alcohol abuse causes widespread changes in the pattern of gene expression in the frontal and motor cortices of human brain. Because these studies used pooled samples, they could not determine variability between different individuals. In the present study, we profiled gene expression levels of 14 postmortem human brains (seven controls and seven alcoholic cases) using cDNA microarrays (46 448 clones per array). Both frontal cortex and motor cortex brain regions were studied. The list of genes differentially expressed confirms and extends previous studies of alcohol responsive genes. Genes identified as differentially expressed in two brain regions fell generally into similar functional groups, including metabolism, immune response, cell survival, cell communication, signal transduction and energy production. Importantly, hierarchical clustering of differentially expressed genes accurately distinguished between control and alcoholic cases, particularly in the frontal cortex.