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PGH2-derived levuglandin adducts increase the neurotoxicity of amyloid β1–42

  1. Olivier Boutaud1,
  2. Thomas J. Montine3,
  3. Lei Chang4,
  4. William L. Klein4,
  5. John A. Oates1,2

Article first published online: 12 JAN 2006

DOI: 10.1111/j.1471-4159.2005.03586.x

Issue

Journal of Neurochemistry

Journal of Neurochemistry

Volume 96, Issue 4, pages 917–923, February 2006

Additional Information

How to Cite

Boutaud, O., Montine, T. J., Chang, L., Klein, W. L. and Oates, J. A. (2006), PGH2-derived levuglandin adducts increase the neurotoxicity of amyloid β1–42. Journal of Neurochemistry, 96: 917–923. doi: 10.1111/j.1471-4159.2005.03586.x

Author Information

  1. 1

    Departments of Pharmacology

  2. 2

    Medicine, Vanderbilt University, Nashville, Tennessee, USA

  3. 3

    Department of Neuropathology, University of Washington, Seattle, Washington, USA

  4. 4

    Department of Neurobiology and Physiology, North-western University, Evanston, Illinois, USA

*Address correspondence and reprint requests to Olivier Boutaud, Ph.D., Division of Clinical Pharmacology, Department of Pharmacology, Vanderbilt University, Nashville, TN 37232–6602, USA. E-mail: olivier.boutaud@mcmail.vanderbilt.edu

Publication History

  1. Issue published online: 26 JAN 2006
  2. Article first published online: 12 JAN 2006
  3. Received July 19, 2005; revised manuscript received October 7, 2005; accepted October 12, 2005.

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Keywords:

  • amyloid;
  • amyloid-derived diffusible ligands;
  • cyclooxygenase;
  • levuglandin;
  • neurotoxicity;
  • prostaglandin H

Abstract

The body of evidence indicating that oligomers of amyloid β1−42 (Aβ1−42) produce toxicity to neurons, together with our demonstration that prostaglandin H2 (PGH2) oligomerizes amyloid β1−42, led to the examination of the neurotoxicity of amyloid β1−42 treated with PGH2. The neurotoxic effects of Aβ1−42 incubated with PGH2 was examined in primary cultures of cerebral neurons of mice, monitoring the reduction of 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as an indicator of cell toxicity. Whereas Aβ1−42 itself, incubated for 24 h, has little or no effect on MTT reduction, Aβ1−42 24 h after exposure to PGH2 produced a marked inhibition of MTT reduction, comparable with the inhibition resulting from Aβ1−42 that has been oligomerized by incubation for 6 days. Similar results were obtained when Aβ1−42 was incubated with levuglandin E2 (LGE2), a reactive aldehyde formed by spontaneous rearrangement of PGH2. The oligomers formed from reaction of Aβ1−42 with LGE2 exhibit immunochemical similarity with amyloid-derived diffusible ligands (ADDLs), as determined by analysis of the products of reaction of Aβ1−42 with LGE2 using western blotting with an antibody that is selective for ADDLs.

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