Neuroprotective role of bradykinin because of the attenuation of pro-inflammatory cytokine release from activated microglia
Version of Record online: 17 NOV 2006
Journal of Neurochemistry
Volume 101, Issue 2, pages 397–410, April 2007
How to Cite
Noda, M., Kariura, Y., Pannasch, U., Nishikawa, K., Wang, L., Seike, T., Ifuku, M., Kosai, Y., Wang, B., Nolte, C., Aoki, S., Kettenmann, H. and Wada, K. (2007), Neuroprotective role of bradykinin because of the attenuation of pro-inflammatory cytokine release from activated microglia. Journal of Neurochemistry, 101: 397–410. doi: 10.1111/j.1471-4159.2006.04339.x
- Issue online: 17 NOV 2006
- Version of Record online: 17 NOV 2006
- Received July 6, 2006; revised manuscript received September 20, 2006; accepted October 11, 2006.
- tumor necrosis factor-alpha
Bradykinin (BK) has been reported to be a mediator of brain damage in acute insults. Receptors for BK have been identified on microglia, the pathologic sensors of the brain. Here, we report that BK attenuated lipopolysaccharide (LPS)-induced release of tumor necrosis factor-alpha (TNF-α) and interleukin-1β from microglial cells, thus acting as an anti-inflammatory mediator in the brain. This effect was mimicked by raising intracellular cAMP or stimulating the prostanoid receptors EP2 and EP4, while it was abolished by a cAMP antagonist, a prostanoid receptor antagonist, or by an inhibitor of the inducible cyclooxygenase (cyclooxygenase-2). BK also enhanced formation of prostaglandin E2 and expression of microsomal prostaglandin E synthase. Expression of BK receptors and EP2/EP4 receptors were also enhanced. Using physiological techniques, we identified functional BK receptors not only in culture, but also in microglia from acute brain slices. BK reduced LPS-induced neuronal death in neuron–microglia co-cultures. This was probably mediated via microglia as it did not affect TNF-α-induced neuronal death in pure neuronal cultures. Our data imply that BK has anti-inflammatory and neuroprotective effects in the central nervous system by modulating microglial function.