Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca2+-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca2+ pumps
Article first published online: 29 JUN 2007
Journal of Neurochemistry
Volume 103, Issue 3, pages 1009–1018, November 2007
How to Cite
Sepúlveda, M. R., Berrocal, M., Marcos, D., Wuytack, F. and Mata, A. M. (2007), Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca2+-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca2+ pumps. Journal of Neurochemistry, 103: 1009–1018. doi: 10.1111/j.1471-4159.2007.04794.x
- Issue published online: 29 JUN 2007
- Article first published online: 29 JUN 2007
- Received March 22, 2007; revised manuscript received May 23, 2007; accepted June 18, 2007.
- calcium pumps;
- Golgi complex and secretory pathway;
- secretory pathway Ca2+-ATPase 1
Membrane fractions of pig cerebellum show Ca2+-ATPase activdity and Ca2+ transport due to the presence of the secretory pathway Ca2+-ATPase (SPCA). The SPCA1 isoform shows a wide distribution in the neurons of pig cerebellum, where it is found in the Golgi complex of the soma of Purkinje, stellate, basket and granule cells, and also in more distal components of the secretory pathway associated with a synaptic localization such as in cerebellar glomeruli. The SPCA1 may be involved in loading the Golgi complex and the secretory vesicles of these specific neuronal cell types with Ca2+ and also Mn2+. This study of the cellular and subcellular localization of SPCA1 pumps relative to the sarco(endo) plasmic reticulum Ca2+-ATPase and plasma membrane Ca2+-ATPase pumps hints to a possible specific role of SPCA1 in controlling the luminal secretory pathway Ca2+ (or Mn2+) levels as well as the local cytosolic Ca2+ levels. In addition, it helps to specify the zones that are most vulnerable to Ca2+ and/or Mn2+ dyshomeostasis, a condition that is held responsible of an increasing number of neurological disorders.