Cell type-specific subunit composition of G protein-gated potassium channels in the cerebellum
Article first published online: 6 DEC 2007
© 2007 The Authors
Journal of Neurochemistry
Volume 105, Issue 2, pages 497–511, April 2008
How to Cite
Aguado, C., Colón, J., Ciruela, F., Schlaudraff, F., Cabañero, M. J., Perry, C., Watanabe, M., Liss, B., Wickman, K. and Luján, R. (2008), Cell type-specific subunit composition of G protein-gated potassium channels in the cerebellum. Journal of Neurochemistry, 105: 497–511. doi: 10.1111/j.1471-4159.2007.05153.x
- Issue published online: 6 DEC 2007
- Article first published online: 6 DEC 2007
- Received October 11, 2007; revised manuscript received November 17, 2007; accepted November 20, 2007.
Vol. 105, Issue 5, 2053–2054, Article first published online: 30 APR 2008
- potassium channel;
- subunit composition
G protein-gated inwardly rectifying potassium (GIRK/Kir3) channels regulate cellular excitability and neurotransmission. In this study, we used biochemical and morphological techniques to analyze the cellular and subcellular distributions of GIRK channel subunits, as well as their interactions, in the mouse cerebellum. We found that GIRK1, GIRK2, and GIRK3 subunits co-precipitated with one another in the cerebellum and that GIRK subunit ablation was correlated with reduced expression levels of residual subunits. Using quantitative RT-PCR and immunohistochemical approaches, we found that GIRK subunits exhibit overlapping but distinct expression patterns in various cerebellar neuron subtypes. GIRK1 and GIRK2 exhibited the most widespread and robust labeling in the cerebellum, with labeling particularly prominent in granule cells. A high degree of molecular diversity in the cerebellar GIRK channel repertoire is suggested by labeling seen in less abundant neuron populations, including Purkinje neurons (GIRK1/GIRK2/GIRK3), basket cells (GIRK1/GIRK3), Golgi cells (GIRK2/GIRK4), stellate cells (GIRK3), and unipolar brush cells (GIRK2/GIRK3). Double-labeling immunofluorescence and electron microscopies showed that GIRK subunits were mainly found at post-synaptic sites. Altogether, our data support the existence of rich GIRK molecular and cellular diversity, and provide a necessary framework for functional studies aimed at delineating the contribution of GIRK channels to synaptic inhibition in the cerebellum.