The present address of Tohru Tezuka is the Medical Top Track Program, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Regulation of dendritic spine morphology by an NMDA receptor-associated Rho GTPase-activating protein, p250GAP
Article first published online: 10 MAR 2008
© 2008 The Authors. Journal Compilation © 2008 International Society for Neurochemistry
Journal of Neurochemistry
Volume 105, Issue 4, pages 1384–1393, May 2008
How to Cite
Nakazawa, T., Kuriu, T., Tezuka, T., Umemori, H., Okabe, S. and Yamamoto, T. (2008), Regulation of dendritic spine morphology by an NMDA receptor-associated Rho GTPase-activating protein, p250GAP. Journal of Neurochemistry, 105: 1384–1393. doi: 10.1111/j.1471-4159.2008.05335.x
- Issue published online: 10 MAR 2008
- Article first published online: 10 MAR 2008
- Received November 26, 2007; revised manuscript received February 22, 2008; accepted February 29, 2008.
Supplementary Fig. 1. p250GAP associates active RhoA and Cdc42 in vitro. HEK293T cells were transfected with expression plasmids for Myc-tagged Rac1 (wild-type and active), Myc-tagged RhoA (wild-type and active), Myc-tagged Cdc42, (wild-type and active), and p250GAP. Two days later, the cells were lysed and anti-Myc immunoprecipitates were incubated with cell lysates expressing p250GAP. Then these immunoprecipitates were subjected to immunoblotting with the indicated antibodies.
Supplementary Fig. 2. p250GAP dissociates from the NR2B subunit of the NMDA receptor following NMDA receptor stimulation (10 μM NMDA, 5 min). p250GAP immunoprecipitates (upper) and cell lysates (lower) from mock-stimulated or NMDA-stimulated cortical neurons (13 DIV) were probed with the indicated antibodies.
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