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Supplementary Fig. 1. p250GAP associates active RhoA and Cdc42 in vitro. HEK293T cells were transfected with expression plasmids for Myc-tagged Rac1 (wild-type and active), Myc-tagged RhoA (wild-type and active), Myc-tagged Cdc42, (wild-type and active), and p250GAP. Two days later, the cells were lysed and anti-Myc immunoprecipitates were incubated with cell lysates expressing p250GAP. Then these immunoprecipitates were subjected to immunoblotting with the indicated antibodies.

Supplementary Fig. 2. p250GAP dissociates from the NR2B subunit of the NMDA receptor following NMDA receptor stimulation (10 μM NMDA, 5 min). p250GAP immunoprecipitates (upper) and cell lysates (lower) from mock-stimulated or NMDA-stimulated cortical neurons (13 DIV) were probed with the indicated antibodies.

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