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Figure S1. Seeding effect of αsyn oligomer type C in SH-SY5Y and BeM17 cells. Immunocytochemical staining of αsyn with ASY-1 antibody (in red) after treatment with different concentrations of unlabeled oligomer type C or solvent controls after overnight incubation (blue, Hoechst 33342 stained nuclei). (a, b) Representative confocal images demonstrate a reduction in cytoplasmic staining of αsyn (in red) and an increase in cell associated aggregates (red spots) in SH-SY5Y stably overexpressing αsyn[A53T] (a) or BeM17 stably over-expressing α-syn[A53T] (b) when treated with different concentrations of oligomer type C.

Figure S2. Confocal z-stack of seeding effect of αsyn oligomer type C in SH-SY5Y cells over-expressing αsyn[A53T]. Immunocytochemical staining of αsyn with ASY-1 antibody (in red) after treatment with 0.1 mg/mL Alexa488 labeled oligomer type C (in green) after overnight incubation. Confocal z-sections showed aggregates (yellow-hued) close to nucleus (blue) in merged images.

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