The present address of Karin M. Danzer is the MassGeneral Institute for Neurodegenerative Disease (MIND), Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
Seeding induced by α-synuclein oligomers provides evidence for spreading of α-synuclein pathology
Article first published online: 4 AUG 2009
© 2009 The Authors. Journal Compilation © 2009 International Society for Neurochemistry
Journal of Neurochemistry
Volume 111, Issue 1, pages 192–203, October 2009
How to Cite
Danzer, K. M., Krebs, S. K., Wolff, M., Birk, G. and Hengerer, B. (2009), Seeding induced by α-synuclein oligomers provides evidence for spreading of α-synuclein pathology. Journal of Neurochemistry, 111: 192–203. doi: 10.1111/j.1471-4159.2009.06324.x
- Issue published online: 14 SEP 2009
- Article first published online: 4 AUG 2009
- Received July 18, 2009; accepted July 28, 2009.
Figure S1. Seeding effect of αsyn oligomer type C in SH-SY5Y and BeM17 cells. Immunocytochemical staining of αsyn with ASY-1 antibody (in red) after treatment with different concentrations of unlabeled oligomer type C or solvent controls after overnight incubation (blue, Hoechst 33342 stained nuclei). (a, b) Representative confocal images demonstrate a reduction in cytoplasmic staining of αsyn (in red) and an increase in cell associated aggregates (red spots) in SH-SY5Y stably overexpressing αsyn[A53T] (a) or BeM17 stably over-expressing α-syn[A53T] (b) when treated with different concentrations of oligomer type C.
Figure S2. Confocal z-stack of seeding effect of αsyn oligomer type C in SH-SY5Y cells over-expressing αsyn[A53T]. Immunocytochemical staining of αsyn with ASY-1 antibody (in red) after treatment with 0.1 mg/mL Alexa488 labeled oligomer type C (in green) after overnight incubation. Confocal z-sections showed aggregates (yellow-hued) close to nucleus (blue) in merged images.
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