The present address of V.J. Appleby is the Children’s Brain Tumour Research Centre, Institute of Genetics, University of Nottingham, Queen’s Medical Centre, Nottingham NG7 2UH, UK.
Disruption of the interaction between myosin VI and SAP97 is associated with a reduction in the number of AMPARs at hippocampal synapses
Article first published online: 6 NOV 2009
© 2009 The Authors. Journal Compilation © 2009 International Society for Neurochemistry
Journal of Neurochemistry
Volume 112, Issue 3, pages 677–690, February 2010
How to Cite
Nash, J. E., Appleby, V. J., Corrêa, S. A. L., Wu, H., Fitzjohn, S. M., Garner, C. C., Collingridge, G. L. and Molnár, E. (2010), Disruption of the interaction between myosin VI and SAP97 is associated with a reduction in the number of AMPARs at hippocampal synapses. Journal of Neurochemistry, 112: 677–690. doi: 10.1111/j.1471-4159.2009.06480.x
- Issue published online: 6 JAN 2010
- Article first published online: 6 NOV 2009
- Received July 6, 2009; revised manuscript received November 3, 2009; accepted November 3, 2009.
- hippocampal neurons;
- myosin VI;
- synaptic plasticity
J. Neurochem. (2010) 112, 677–690.
Myosin VI is an actin-based motor protein that is enriched at the postsynaptic density and appears to interact with alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate-type glutamate receptors (AMPARs) via synapse associated protein 97 (SAP97). Here, we find that a Flag epitope-tagged dominant negative construct that inhibits the interaction between SAP97 and myosin VI (Flag-myoVI-DN) causes a dramatic reduction in the number of synapses and the surface expression of AMPARs in cultured hippocampal neurons. Furthermore, we find that Flag-myoVI-DN also prevents the rapid delivery of AMPARs to synapses that can be induced by the transient activation of N-methyl-d-aspartate receptors. The Flag-myoVI-DN induced decrease in surface AMPARs is not because of reduced AMPAR subunit protein synthesis. Using whole-cell recording, we show that Flag-myoVI-DN also prevents the activity-induced increase in miniature excitatory postsynaptic current frequency that is normally associated with recruitment of AMPARs to the cell surface at synaptic sites that lack these receptors (i.e. ‘silent’ synapses). Together, these results indicate that myosin VI/SAP97 plays an important role in trafficking and activity-dependent recruitment of AMPARs to synapses.