These authors contributed equally to this study.
Studies on the immune response and preparation of antibodies against a large panel of conjugated neurotransmitters and biogenic amines: specific polyclonal antibody response and tolerance
Article first published online: 13 NOV 2009
© 2009 Pharmasan Labs Inc. Journal Compilation © 2009 International Society for Neurochemistry
Journal of Neurochemistry
Volume 112, Issue 3, pages 829–841, February 2010
How to Cite
Huisman, H., Wynveen, P. and Setter, P. W. (2010), Studies on the immune response and preparation of antibodies against a large panel of conjugated neurotransmitters and biogenic amines: specific polyclonal antibody response and tolerance. Journal of Neurochemistry, 112: 829–841. doi: 10.1111/j.1471-4159.2009.06492.x
- Issue published online: 6 JAN 2010
- Article first published online: 13 NOV 2009
- Received October 22, 2009; accepted November 9, 2009.
- biogenic amines;
- competitive ELISA;
- detection in situ;
- polyclonal antibodies;
J. Neurochem. (2010) 112, 840–852.
We described the production and characterization of antibodies against three important groups of neuro-active haptens, e.g., neurotransmitters and biogenic amines. First, from the tryptophane metabolic pathway: tryptamine, serotonin, 5-hydroxy-indole acetic acid, and melatonin. Secondly, the tyrosine metabolic pathway: tyramine, dopamine, dihydroxyphenyl acetic acid, and norepinephrine. Thirdly, antibodies against excitatory and inhibitory neurotransmitters: glycine, glutamate, glutamine, and GABA. Immunogenic conjugates were prepared after linking haptens to carrier proteins. Most antibodies displayed high specificity against corresponding neuro-active haptens conjugated in vitro and in situ in biological specimens, but not to closely related conjugated metabolites, precursors, pharmaceuticals, agonists, antagonists, or free neuro-active haptens. Conjugated norepinephrine was highly tolerant in different animal species and produced incidentally a short specific antibody response.