Mitochondrial respiration and respiration-associated proteins in cell lines created through Parkinson’s subject mitochondrial transfer
Version of Record online: 1 FEB 2010
© 2010 The Authors. Journal Compilation © 2010 International Society for Neurochemistry
Journal of Neurochemistry
Volume 113, Issue 3, pages 674–682, May 2010
How to Cite
Esteves, A. R., Lu, J., Rodova, M., Onyango, I., Lezi, E., Dubinsky, R., Lyons, K. E., Pahwa, R., Burns, J. M., Cardoso, S. M. and Swerdlow, R. H. (2010), Mitochondrial respiration and respiration-associated proteins in cell lines created through Parkinson’s subject mitochondrial transfer. Journal of Neurochemistry, 113: 674–682. doi: 10.1111/j.1471-4159.2010.06631.x
- Issue online: 6 APR 2010
- Version of Record online: 1 FEB 2010
- Received November 19, 2009; revised manuscript received January 20, 2010; accepted January 21, 2010.
- Parkinson’s disease;
J. Neurochem. (2010) 113, 674–682.
Parkinson’s disease (PD) is associated with perturbed mitochondrial function. Studies of cytoplasmic hybrid (cybrid) cell lines containing mitochondria from PD subjects suggest complex I dysfunction in particular is a relatively upstream biochemical defect. To evaluate potential downstream consequences of PD mitochondrial dysfunction, we used a cybrid approach to model PD mitochondrial dysfunction; our cybrid cell lines were generated via transfer of PD or control subject platelet mitochondria to mtDNA-depleted NT2 cells. To confirm our PD cybrid mitochondria did indeed differ from control cybrid mitochondria we measured complex I Vmax activities. Consistent with other PD cybrid reports, relative to control cybrid cell lines the PD cybrid cell line mean complex I Vmax activity was reduced. In this validated model, we used an oxygen electrode to characterize PD cybrid mitochondrial respiration. Although whole cell basal oxygen consumption was comparable between the PD and control cybrid groups, the proton leak was increased and maximum respiratory capacity was decreased in the PD cybrids. PD cybrids also had reduced SIRT1 phosphorylation, reduced peroxisome proliferator-activated receptor-γ coactivator-1α levels, and increased NF-kB activation. We conclude mitochondrial respiration and pathways influenced by aerobic metabolism are altered in NT2 cybrid cell lines generated through transfer of PD subject platelet mitochondria.