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Appendix S1. Supplementary Materials and Methods.

Figure S1. Line graph illustrating time course changes of (a) tcpCO2 and (b) tcpO2 following subcutaneous administration of vehicle, or 2-DG (200 mg/kg) in α-chloralose anaesthetized rats (n = 7). An initial 15-min baseline period was acquired before drugs were injected (vertical dotted line). Results are presented as percentage change relative to baseline values (100%). Horizontal dotted line represents threshold level as described by Cabrer et al. (2005) by which BOLD signal is lost if tcpCO2 levels exceed 20% above baseline (Ramos-Cabrer et al. 2005). Error bars show SEM.

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