Katja Schneider and Susanne Tippmann share first authorship.
Unique clockwork in photoreceptor of rat
Article first published online: 27 SEP 2010
© 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry
Journal of Neurochemistry
Volume 115, Issue 3, pages 585–594, November 2010
How to Cite
Schneider, K., Tippmann, S., Spiwoks-Becker, I., Holthues, H., Wolloscheck, T., Spatkowski, G., Engel, L., Frederiksen, U. and Spessert, R. (2010), Unique clockwork in photoreceptor of rat. Journal of Neurochemistry, 115: 585–594. doi: 10.1111/j.1471-4159.2010.06953.x
- Issue published online: 13 OCT 2010
- Article first published online: 27 SEP 2010
- Accepted manuscript online: 12 AUG 2010 12:00AM EST
- Received May 20, 2010; revised manuscript received July 15, 2010; accepted July 27, 2010.
- arylalkylamine N-acetyltransferase;
- clock genes;
J. Neurochem. (2010) 115, 585–594.
In mammals, the retina contains a clock system that oscillates independently of the master clock in the suprachiasmatic nucleus and allows the retina to anticipate and to adapt to the sustained daily changes in ambient illumination. Using a combination of laser capture micro-dissection and quantitative PCR in the present study, the clockwork of mammalian photoreceptors has been recorded. The transcript amounts of the core clock genes Clock, Bmal1, Period1 (Per1), Per3, Cryptochrome2, and Casein kinase Iε in photoreceptors of rat retina have been found to undergo daily changes. Clock and Bmal1 peak with Per1 and Per3 around dark onset, whereas Casein kinase Iε and Cryptochrome2 peak at night. As shown for Clock, Per1, and Casein kinase Iε, the oscillation of transcript amounts results in daily changes of the protein products. The in-phase oscillation of Clock/Bmal1 with Pers and the rhythmic expression of Casein kinase Iε do not occur in molecular clocks of other tissues including the suprachiasmatic nucleus. Therefore, the findings presented suggest that the photoreceptor clock is unique not only in its position outside the clock hierarchy mastered by the suprachiasmatic nucleus, but also with regard to the intrinsic rhythmic properties of its molecular components.