Figure S1. (a) N2a cells were stimulated with 1 μM FK506 for 3 h or 24 h. The right panel (Aged) corresponds to incubations performed with a medium reused in a previous incubation of 72 h. (b) Toxic effects of cyclosporine A (CsA) or radicicol (RAD) can also be seen after 24 h of incubation with concentrations five times lower than those used in Fig. 1(a). Cells treated with 1 μM FK506 for the same period of time are shown for comparative purposes.

Figure S2. Bar graph for neurite lengths measured in identical conditions as those described for Fig. 2(a) except that the incubation time was 24 h.

Figure S3. Subcellular distribution chaperones in 3T3-L1 pre-adipocytes is not affected by 4-h treatment with a differentiation cocktail. Bar = 10 μm.

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