Neurochemical profile of the developing mouse cortex determined by in vivo1H NMR spectroscopy at 14.1 T and the effect of recurrent anaesthesia
Version of Record online: 4 NOV 2010
© 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry
Journal of Neurochemistry
Volume 115, Issue 6, pages 1466–1477, December 2010
How to Cite
Kulak, A., Duarte, J. M. N., Do, K. Q. and Gruetter, R. (2010), Neurochemical profile of the developing mouse cortex determined by in vivo1H NMR spectroscopy at 14.1 T and the effect of recurrent anaesthesia. Journal of Neurochemistry, 115: 1466–1477. doi: 10.1111/j.1471-4159.2010.07051.x
- Issue online: 1 DEC 2010
- Version of Record online: 4 NOV 2010
- Accepted manuscript online: 14 OCT 2010 11:54AM EST
- Received June 15, 2010; revised manuscript received September 27, 2010; accepted October 5, 2010.
- cerebral cortex;
- NMR spectroscopy
J. Neurochem. (2010) 115, 1466–1477.
The neurochemical profile of the cortex develops in a region and time specific manner, which can be distorted by psychiatric and other neurological pathologies. Pre-clinical studies often involve experimental mouse models. In this study, we determined the neurochemical profile of C57BL/6 mice in a longitudinal study design to provide a reference frame for the normal developing mouse cortex. Using in vivo proton NMR spectroscopy at 14 T, we measured the concentrations of 18 metabolites in the anterior and posterior cortex on postnatal days (P) 10, 20, 30, 60 and 90. Cortical development was marked by alterations of highly concentrated metabolites, such as N-acetylaspartate, glutamate, taurine and creatine. Regional specificity was represented by early variations in the concentration of glutamine, aspartate and choline. In adult animals, regional concentration differences were found for N-acetylaspartate, creatine and myo-inositol. In this study, animals were exposed to recurrent isoflurane anaesthesia. Additional experiments showed that the latter was devoid of major effects on behaviour or cortical neurochemical profile. In conclusion, the high sensitivity and reproducibility of the measurements achieved at 14 T allowed us to identify developmental variations of cortical areas within the mouse cortex.