New approach for glyco- and lipidomics – Molecular scanning of human brain gangliosides by TLC-Blot and MALDI-QIT-TOF MS

Authors

  • Tania Valdes-Gonzalez,

    1. Third Institute of New Drug Discovery, Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., Kagasuno, Kawauchi-cho, Tokushima, Japan
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    • These authors contributed equally to this work.

  • Naoko Goto-Inoue,

    1. Department of Molecular Anatomy, Molecular Imaging, Frontier Research Center, Hamamatsu University School of Medicine, Handayama, Higashi-ku Hamamatsu, Shizuoka, Japan
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    • These authors contributed equally to this work.

  • Wakako Hirano,

    1. Third Institute of New Drug Discovery, Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., Kagasuno, Kawauchi-cho, Tokushima, Japan
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  • Hironobu Ishiyama,

    1. Third Institute of New Drug Discovery, Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., Kagasuno, Kawauchi-cho, Tokushima, Japan
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  • Takahiro Hayasaka,

    1. Department of Molecular Anatomy, Molecular Imaging, Frontier Research Center, Hamamatsu University School of Medicine, Handayama, Higashi-ku Hamamatsu, Shizuoka, Japan
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  • Mitsutoshi Setou,

    1. Department of Molecular Anatomy, Molecular Imaging, Frontier Research Center, Hamamatsu University School of Medicine, Handayama, Higashi-ku Hamamatsu, Shizuoka, Japan
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  • Takao Taki

    1. Third Institute of New Drug Discovery, Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., Kagasuno, Kawauchi-cho, Tokushima, Japan
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Address correspondence and reprint requests to Takao Taki, Third Institute of New Drug Discovery, Institute of Biomedical Innovation; Otsuka Pharmaceutical Co. Ltd. 463-10 Kagasuno, Kawauchi-cho, Tokushima, 771-0192 Japan. E-mail:t_taki@research.otsuka.co.jp

Abstract

J. Neurochem. (2011) 116, 678–683.

Abstract

We have developed a TLC-Blot system that makes possible the direct analysis of blotted glycosphingolipids on a polyvinylidene difluoride membrane from a high-performance TLC plate by immunological staining, chemical staining, enzymatic treatment and mass spectrometric (MS) analysis. An ion trap type matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight (MALDI-QIT-TOF) MS apparatus improved not only the molecular identification but also the analysis of molecular species of lipids on the polyvinylidene difluoride membrane. A new approach for glyco- and lipidomics, molecular scanning technology by a combination of TLC-Blot and MALDI-QIT-TOF MS, was developed and applied to human brain gangliosides separated from the tissues of patients with neural diseases and control patients. The results clearly showed a change of ganglioside composition, in addition to identifying individual ganglioside molecular species, in the hippocampus gray matter of patients with Alzheimer’s disease. The results strongly suggested that metabolic changes of gangliosides played an important role in the progression of this disease. The present technology with molecular imaging should provide valuable information for elucidating the significance of molecular species in neuronal functions such as neural transmission, memory, and learning.

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