These authors contributed equally to this work.
New approach for glyco- and lipidomics – Molecular scanning of human brain gangliosides by TLC-Blot and MALDI-QIT-TOF MS
Article first published online: 19 JAN 2011
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry
Journal of Neurochemistry
Special Issue: Fourth ISN Special Conference 'Membrane Domains in CNS Physiology and Pathology', Erice (Trapani), Sicily, Italy, 22-26 May 2010
Volume 116, Issue 5, pages 678–683, March 2011
How to Cite
Valdes-Gonzalez, T., Goto-Inoue, N., Hirano, W., Ishiyama, H., Hayasaka, T., Setou, M. and Taki, T. (2011), New approach for glyco- and lipidomics – Molecular scanning of human brain gangliosides by TLC-Blot and MALDI-QIT-TOF MS. Journal of Neurochemistry, 116: 678–683. doi: 10.1111/j.1471-4159.2010.07152.x
- Issue published online: 9 FEB 2011
- Article first published online: 19 JAN 2011
- Received September 2, 2010; revised manuscript received December 8, 2010; accepted December 10, 2010.
- Alzheimer’s disease;
- MALD-QIT-TOF MS/TLC-Blot
J. Neurochem. (2011) 116, 678–683.
We have developed a TLC-Blot system that makes possible the direct analysis of blotted glycosphingolipids on a polyvinylidene difluoride membrane from a high-performance TLC plate by immunological staining, chemical staining, enzymatic treatment and mass spectrometric (MS) analysis. An ion trap type matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight (MALDI-QIT-TOF) MS apparatus improved not only the molecular identification but also the analysis of molecular species of lipids on the polyvinylidene difluoride membrane. A new approach for glyco- and lipidomics, molecular scanning technology by a combination of TLC-Blot and MALDI-QIT-TOF MS, was developed and applied to human brain gangliosides separated from the tissues of patients with neural diseases and control patients. The results clearly showed a change of ganglioside composition, in addition to identifying individual ganglioside molecular species, in the hippocampus gray matter of patients with Alzheimer’s disease. The results strongly suggested that metabolic changes of gangliosides played an important role in the progression of this disease. The present technology with molecular imaging should provide valuable information for elucidating the significance of molecular species in neuronal functions such as neural transmission, memory, and learning.