Tetraspanin, CD151, is required for maintenance of trigeminal placode identity
Article first published online: 24 FEB 2011
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry
Journal of Neurochemistry
Volume 117, Issue 2, pages 221–230, April 2011
How to Cite
McCabe, K. L. and Bronner, M. (2011), Tetraspanin, CD151, is required for maintenance of trigeminal placode identity. Journal of Neurochemistry, 117: 221–230. doi: 10.1111/j.1471-4159.2011.07190.x
- Issue published online: 1 APR 2011
- Article first published online: 24 FEB 2011
- Accepted manuscript online: 19 JAN 2011 11:49AM EST
- Received October 13, 2010; revised manuscript received December 10, 2010; accepted January 6, 2011.
- trigeminal placode
J. Neurochem. (2011) 117, 221–230.
The trigeminal ganglion is the largest of the cranial ganglia and responsible for transmitting sensory information for much of the face. The cell surface glycoprotein CD151 is an early marker of the trigeminal placode, the precursor to the ganglion. Here, we investigate the role of CD151 during specification of trigeminal placode cells in the developing chicken embryo. Expression of the transcription factor Pax3, the earliest known marker of the trigeminal placode, briefly precedes that of CD151, but they then subsequently overlap in the trigeminal placode. Loss of CD151 protein dramatically decreases the number of Pax3+ placode cells in Stage 13–14 embryos, leading to loss of ophthalmic trigeminal neurons by Stages 16 and 17. Although the initial size of the Pax3 population is similar to that in controls, the number of Pax3+ cells decreases with time without alterations in cell death or proliferation. This suggests a role for CD151 in maintenance of the specification state in the trigeminal placode, uncovering the first known role for a tetraspanin in a developmental system.