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Appendix S1. Supplementary method.

Figure S1. Fatty acids identified by GC-MS from isolated zebra finch brain tissue.

Figure S2. Control ToF-SIMS analysis of showing ion spectra from m/z 200–800 using a 99% pure sample of phosphatidylethanolamine (PE).

Figure S3. Anatomical analysis across AreaX, DLM, HVC, and LMAN and the distribution of AA and DHA.

Figure S4. Fatty acid  distribution across the auditory lobule.

Figure S5. ToF-SIMS images showing the relative distribution of various non-essential fatty acids across different song nuclei in the zebra finch brain.

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