Figure S1. Imaging of mitochondrial ROS production in situ. PC12 cells grown on glass cover slips were stained with MitoSOXred (5 μM) for 30 min. After washing (twice), cells were treated either with 20 μM arachidonic acid (Ara) or 5 μM antimycin A (AA; inhibitor of complex III) for 30 min. Thereafter, cells were washed again, and finally fixed with 1% paraformaldehyde in PBS. Left column: pseudo color image representing the red fluorescence of the oxidized MitoSOXred probe. Right column: overlay of the red fluorescence channel with the respective differential interference contrast images showing the cells.

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