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Figure S1. Characterization of neuronal cell-enriched cultures from embryonic striatal tissues. Cultures were immunostained with anti-MAP2 antibody (a), anti-NeuN antibody (b), anti-GFAP antibody (c) and anti-nestin antibody (d) followed by anti-mouse or anti-rabbit immunoglobulin fluorescent secondary antibodies. Left panels represent the phase contrasted microscopic views corresponding to the right panels. The frequency of individual cell types is shown in Table 1 (= 4 sister cultures). Representative pictures are displayed. Scale bar, 100 μm.

Figure S2. Protein samples were prepared from neuron-enriched and non-neuronal cell-enriched culture, subjected to immunoblotting with the anti-dopamine receptor antibodies, and compared with the levels in the striatum of postnatal rats (PND7). Alternatively, the immunoblots were probed with anti-neuron-specific enolase (NSE) antibody or anti-glial fibrillary acidic protein (GFAP) antibody. Representative two lanes of the immunoblot are shown for figure display.

Figure S3. Characterization of non-neuronal cell-enriched cultures from embryonic striatal tissues. Cultures were immunostained with the anti-A2B5 antibody (a), anti-GFAP antibody (b), anti-MAP2 antibody (c) and anti-nestin antibody (d) followed by anti-mouse or anti-rabbit immunoglobulin fluorescent secondary antibodies. Left panels represent the phase contrasted microscopic views corresponding to the right panels. The frequency of individual cell types is shown in Table 2 (= 4 sister cultures). Representative pictures are displayed. Scale bars, 100 μm.

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