Protective effect of the octadecaneuropeptide on hydrogen peroxide-induced oxidative stress and cell death in cultured rat astrocytes

Authors

  • Yosra Hamdi,

    1. Laboratory of Functional Neurophysiology and Pathology, Research Unit and 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia
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    • These authors contributed equally to this study.

  • Olfa Masmoudi-Kouki,

    1. Laboratory of Functional Neurophysiology and Pathology, Research Unit and 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia
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    • These authors contributed equally to this study.

  • Hadhemi Kaddour,

    1. Laboratory of Functional Neurophysiology and Pathology, Research Unit and 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia
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  • Feten Belhadj,

    1. Biological Engineering Unit 99UR09-26, National Institute of Applied Sciences and Technology, Tunis, Tunisia
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  • Pierrick Gandolfo,

    1. Inserm U413/U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, Mont-Saint-Aignan, France
    2. International Associated Laboratory Samuel de Champlain, University of Rouen, Mont-Saint-Aignan, France
    3. European Institute for Peptide Research (IFRMP 23), Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), University of Rouen, Mont-Saint-Aignan, France
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  • David Vaudry,

    1. Inserm U413/U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, Mont-Saint-Aignan, France
    2. International Associated Laboratory Samuel de Champlain, University of Rouen, Mont-Saint-Aignan, France
    3. European Institute for Peptide Research (IFRMP 23), Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), University of Rouen, Mont-Saint-Aignan, France
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  • Meherzia Mokni,

    1. Laboratory of Functional Neurophysiology and Pathology, Research Unit and 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia
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  • Jérôme Leprince,

    1. Inserm U413/U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, Mont-Saint-Aignan, France
    2. International Associated Laboratory Samuel de Champlain, University of Rouen, Mont-Saint-Aignan, France
    3. European Institute for Peptide Research (IFRMP 23), Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), University of Rouen, Mont-Saint-Aignan, France
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  • Raya Hachem,

    1. Laboratory of Functional Neurophysiology and Pathology, Research Unit and 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia
    2. Inserm U413/U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, Mont-Saint-Aignan, France
    3. International Associated Laboratory Samuel de Champlain, University of Rouen, Mont-Saint-Aignan, France
    4. European Institute for Peptide Research (IFRMP 23), Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), University of Rouen, Mont-Saint-Aignan, France
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  • Hubert Vaudry,

    1. Inserm U413/U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, Mont-Saint-Aignan, France
    2. International Associated Laboratory Samuel de Champlain, University of Rouen, Mont-Saint-Aignan, France
    3. European Institute for Peptide Research (IFRMP 23), Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), University of Rouen, Mont-Saint-Aignan, France
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  • Marie-Christine Tonon,

    1. Inserm U413/U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, Mont-Saint-Aignan, France
    2. International Associated Laboratory Samuel de Champlain, University of Rouen, Mont-Saint-Aignan, France
    3. European Institute for Peptide Research (IFRMP 23), Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), University of Rouen, Mont-Saint-Aignan, France
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  • Mohamed Amri

    1. Laboratory of Functional Neurophysiology and Pathology, Research Unit and 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia
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Address correspondence and reprint requests to Pr Mohamed Amri, Laboratory of Functional Neurophysiology and Pathology, 00/UR/08-01, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, 2092 Tunis, Tunisia. E-mail: mohamed.amri@fst.rnu.tn and Dr Hubert Vaudry, Inserm U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, International Associated Laboratory Samuel de Champlain, Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), European Institute for Peptide Research (IFRMP 23), University of Rouen, 76821 Mont-Saint-Aignan, France. E-mail: hubert.vaudry@univ-rouen.fr

Abstract

J. Neurochem. (2011) 118, 416–428.

Abstract

Oxidative stress, resulting from accumulation of reactive oxygen species (ROS), plays a critical role on astrocyte death associated with neurodegenerative diseases. Astroglial cells produce endozepines, a family of biologically active peptides that have been implicated in cell protection. Thus, the purpose of the present study was to investigate the potential protective effect of one of the endozepines, the octadecaneuropeptide ODN, on hydrogen peroxide (H2O2)-induced oxidative stress and cell death in rat astrocytes. Incubation of cultured astrocytes with graded concentrations of H2O2 for 1 h provoked a dose-dependent reduction of the number of living cells as evaluated by lactate dehydrogenase assay. The cytotoxic effect of H2O2 was associated with morphological modifications that were characteristic of apoptotic cell death. H2O2-treated cells exhibited high level of ROS associated with a reduction of both superoxide dismutases (SOD) and catalase activities. Pre-treatment of astrocytes with low concentrations of ODN dose-dependently prevented cell death induced by H2O2. This effect was accompanied by a marked attenuation of ROS accumulation, reduction of mitochondrial membrane potential and activation of caspase 3 activity. ODN stimulated SOD and catalase activities in a concentration-dependent manner, and blocked H2O2-evoked inhibition of SOD and catalase activities. Blockers of SOD and catalase suppressed the effect of ODN on cell survival. Taken together, these data demonstrate for the first time that ODN is a potent protective agent that prevents oxidative stress-induced apoptotic cell death.

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