Cross-talk between human mesenchymal stem/progenitor cells (MSCs) and rat hippocampal slices in LPS-stimulated cocultures: the MSCs are activated to secrete prostaglandin E2

Authors

  • Jessica E. Foraker,

    1. Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA
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  • Joo Youn Oh,

    1. Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA
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  • Joni H. Ylostalo,

    1. Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA
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  • Ryang Hwa Lee,

    1. Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA
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  • Jun Watanabe,

    1. Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA
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  • Darwin J. Prockop

    1. Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA
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Address correspondence and reprint requests to Prockop D. J., Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, 5701 Airport Rd, Module C, Temple, TX 76502, USA. E-mail: prockop@medicine.tamhsc.edu

Abstract

J. Neurochem. (2011) 119, 1052–1063.

Abstract

Mesenchymal stem/progenitor cells (MSCs) improve functional outcome in a number of disease models through suppression of inflammation. However, their effects on neuroinflammation are unknown. In this study, we show that MSCs suppress endotoxin-induced glial activation in organotypic hippocampal slice cultures (OHSCs). Lipopolysaccharide-stimulated OHSCs activated MSCs to increase the expression of cyclo-oxygenase-2 and produce prostaglandin E2. MSC-derived prostaglandin E2, then suppressed pro-inflammatory cytokine production by the OHSCs. Together, the results suggest the potential anti-inflammatory mechanism of MSCs in models of disease and support earlier observations that MSCs may offer a therapy for neuroinflammation produced by trauma or disease.

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