These authors contributed equally to this work.
Identification of differentially expressed microRNAs and their PKC-isoform specific gene network prediction during hypoxic pre-conditioning and focal cerebral ischemia of mice
Article first published online: 23 JAN 2012
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry
Journal of Neurochemistry
Volume 120, Issue 5, pages 830–841, March 2012
How to Cite
Liu, C., Peng, Z., Zhang, N., Yu, L., Han, S., Li, D. and Li, J. (2012), Identification of differentially expressed microRNAs and their PKC-isoform specific gene network prediction during hypoxic pre-conditioning and focal cerebral ischemia of mice. Journal of Neurochemistry, 120: 830–841. doi: 10.1111/j.1471-4159.2011.07624.x
- Issue published online: 10 FEB 2012
- Article first published online: 23 JAN 2012
- Accepted manuscript online: 15 DEC 2011 11:47PM EST
- Received October 15, 2011; revised manuscript received December 6, 2011; accepted December 12, 2011.
- gene ontology;
- hypoxic pre-conditioning;
- Kyoto encyclopedia of genes and genomes;
- miRNA microarray;
- middle cerebral artery occlusion
J. Neurochem. (2012) 120, 830–841.
We previously reported the involvement of conventional protein kinase C (cPKC) βII, γ, novel PKC (nPKC) ε and their interacting proteins in hypoxic pre-conditioning (HPC)-induced neuroprotection. In this study, the large-scale miRNA microarrays and bioinformatics analysis were used to determine the differentially expressed miRNAs and their PKC-isoform specific gene network in mouse brain after HPC and 6 h middle cerebral artery occlusion (MCAO). We found 4 up-regulated and 13 down-regulated miRNAs in the cortex of HPC mice, 26 increased and 39 decreased gene expressions of miRNAs in the peri-infarct region of 6 h MCAO mice, and 11 up-regulated and 22 down-regulated miRNAs in the peri-infarct region of HPC and 6 h MCAO mice. Based on Diff Score, 19 differentially expressed miRNAs were identified in HPC and 6 h MCAO mouse brain. Then the miRNA-gene-network of 19 specified miRNAs target genes of cPKCβII, γ and nPKCε-interacting protein was predicted by using bioinformatics analysis of genome databases. Furthermore, the down-regulated miR-615-3p during HPC had a detrimental effect on the oxygen-glucose deprivation (OGD)-induced N2A cell injury. These results suggested that the identified 19 miRNAs, notably miR-615-3p, might target these genes of cPKCβII, γ and nPKCε-interacting proteins involved in HPC-induced neuroprotection.