These authors contributed equally to this work.
Identification of differentially expressed microRNAs and their PKC-isoform specific gene network prediction during hypoxic pre-conditioning and focal cerebral ischemia of mice
Article first published online: 23 JAN 2012
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry
Journal of Neurochemistry
Volume 120, Issue 5, pages 830–841, March 2012
How to Cite
Liu, C., Peng, Z., Zhang, N., Yu, L., Han, S., Li, D. and Li, J. (2012), Identification of differentially expressed microRNAs and their PKC-isoform specific gene network prediction during hypoxic pre-conditioning and focal cerebral ischemia of mice. Journal of Neurochemistry, 120: 830–841. doi: 10.1111/j.1471-4159.2011.07624.x
- Issue published online: 10 FEB 2012
- Article first published online: 23 JAN 2012
- Accepted manuscript online: 15 DEC 2011 11:47PM EST
- Received October 15, 2011; revised manuscript received December 6, 2011; accepted December 12, 2011.
Figure S1. Representative electrophoretogram of total RNA extracted from cerebral cortex or peri-infarct region of mice. Two bands of 28S and 18S ribosomal RNA could be found in normoxic control, hypoxic preconditioning (HPC), MCAO-induced focal ischemia (I) and HPC+I groups (n = 12 per group).
Figure S2. OGD induced cell injury and modulated miR-615-3p expression in cultured N2A cells. N2A cells were exposed to OGD for 2 or 4 h and reoxygenation for 24 h. N2A cell injuries were detected by MTT (A) and LDH (B) assays (*p < 0.05 vs normoxic control, n = 6 per group). (C) miR-615-3p levels were determined by qRT-PCR after transfection of vehicle control, pre-miR control, pre-miR-615-3p, anti-miR control, or anti-miR-615-3p at a final concentration of 30 nM for 48 h (*p < 0.05 vs vehicle control, n = 6 per group).
Table S1. Original data of all the 655 probes in cortex of normoxic control and HPC mice (n = 12 per group)
Table S2. Original data of all the 655 probes in cortex of the sham control and peri-infarct region of 6 h MCAO mice (n = 12 per group)
Table S3. Original data of all the 655 probes in peri-infarct region of 6 h MCAO (I) and HPC+I mice (n = 12 per group)
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