Effects of acute and chronic hyperglycemia on the neurochemical profiles in the rat brain with streptozotocin-induced diabetes detected using in vivo1H MR spectroscopy at 9.4 T
Article first published online: 14 MAR 2012
© 2012 The Authors. Journal of Neurochemistry © 2012 International Society for Neurochemistry
Journal of Neurochemistry
Volume 121, Issue 3, pages 407–417, May 2012
How to Cite
Wang, W.-T., Lee, P., Yeh, H.-W., Smirnova, I. V. and Choi, I.-Y. (2012), Effects of acute and chronic hyperglycemia on the neurochemical profiles in the rat brain with streptozotocin-induced diabetes detected using in vivo1H MR spectroscopy at 9.4 T. Journal of Neurochemistry, 121: 407–417. doi: 10.1111/j.1471-4159.2012.07698.x
- Issue published online: 10 APR 2012
- Article first published online: 14 MAR 2012
- Accepted manuscript online: 21 FEB 2012 11:50AM EST
- Received September 26, 2011; revised manuscript received February 7, 2012; accepted February 16, 2012.
- disease staging;
- glucose transport;
- magnetic resonance spectroscopy;
- uncontrolled hyperglycemia
J. Neurochem. (2012) 121, 407–417.
Chronic hyperglycemia could lead to cerebral metabolic alterations and CNS injury. However, findings of metabolic alterations in poorly managed diabetes in humans and animal models are rather inconsistent. We have characterized the cerebral metabolic consequences of untreated hyperglycemia from the onset to the chronic stage in a streptozotocin-induced rat model of diabetes. In vivo1H magnetic resonance spectroscopy was used to measure over 20 neurochemicals longitudinally. Upon the onset of hyperglycemia (acute state), increases in brain glucose levels were accompanied by increases in osmolytes and ketone bodies, all of which remained consistently high through the chronic state of over 10 weeks of hyperglycemia. Only after over 4 weeks of hyperglycemia, the levels of other neurochemicals including N-acetylaspartate and glutathione were significantly reduced and these alterations persisted into the chronic stage. However, glucose transport was not altered in chronic hyperglycemia of over 10 weeks. When glucose levels were acutely restored to euglycemia, some neurochemical changes were irreversible, indicating the impact of prolonged uncontrolled hyperglycemia on the CNS. Furthermore, progressive changes in neurochemical levels from control to acute and chronic conditions demonstrated the utility of 1H magnetic resonance spectroscopy as a non-invasive tool in monitoring the disease progression in diabetes.