These authors contributed equally to this work.
Dopamine D1 receptors regulate type 1 inositol 1,4,5-trisphosphate receptor expression via both AP-1- and NFATc4-mediated transcriptional processes
Article first published online: 9 JUL 2012
© 2012 The Authors. Journal of Neurochemistry © 2012 International Society for Neurochemistry
Journal of Neurochemistry
Volume 122, Issue 4, pages 702–713, August 2012
How to Cite
Mizuno, K., Kurokawa, K. and Ohkuma, S. (2012), Dopamine D1 receptors regulate type 1 inositol 1,4,5-trisphosphate receptor expression via both AP-1- and NFATc4-mediated transcriptional processes. Journal of Neurochemistry, 122: 702–713. doi: 10.1111/j.1471-4159.2012.07827.x
- Issue published online: 17 JUL 2012
- Article first published online: 9 JUL 2012
- Accepted manuscript online: 11 JUN 2012 01:30PM EST
- Received April 12, 2012; revised manuscript received May 17, 2012; accepted June 4, 2012.
- dopamine D1 receptors;
- type 1 inositol 1,4,5-trisphosphate receptors
J. Neurochem. (2012) 122, 702–713.
Although our recent report demonstrates the essential involvement of up-regulation of a regulator of intracellular Ca2+ concentration, type 1 inositol 1,4,5-trisphosphate receptors (IP3Rs-1), mediated via dopamine D1-like receptor (D1DR) stimulation in the cocaine-induced psychological dependence, the exact mechanisms of regulation of IP3R-1 expression by D1DRs have not yet been clarified. This study attempted to clarify these mechanisms using mouse cerebral cortical neurons. An agonist for phosphatidylinositide-linked D1DRs, SKF83959, induced dose- and time-dependently IP3R-1 protein up-regulation following its mRNA increase without cAMP production. U73122 (a phospholipase C inhibitor), BAPTA-AM (an intracellular calcium chelating reagent), W7 (a calmodulin inhibitor), KN-93 (a calmodulin-dependent protein kinases inhibitor), and FK506 (a calcineurin inhibitor), significantly inhibited the SKF83959-induced IP3R-1 up-regulation. Furthermore, immunohistochemical examinations showed that SKF83959 increased expression of both cFos and cJun in nucleus as well as enhanced translocation of both calcineurin and NFATc4 complex to nucleus from cytoplasm. In addition, SKF83959 directly recruited binding of both AP-1 and NFATc4 to IP3R-1 promoter region. These results indicate that D1DR activation induces IP3R-1 up-regulation via increased translocation of AP-1 as well as NFATc4 in Gαq protein-coupled calcium signaling transduction pathway.