Stereotype threat research has shown that being a member of a negatively stereotyped group may result in impaired performance on tests of skills thought to be relevant to the stereotype. This study investigated whether stereotype threat influences gender differences in performance on a novel test of visuospatial ability. Undergraduates (N = 194) were told that men outperform women on the test (explicit threat), were given no gender-relevant information (implicit threat), or were told that men and women do not differ (nullified stereotype). Although men outperformed women in the explicit and implicit stereotype threat groups, women's performance did not differ significantly from men's when told there is no gender difference. The effect was most pronounced for difficult line judgments. Although stereotypes regarding visuospatial ability may be less culturally salient than those of other cognitive abilities, these findings suggest that they influence performance nonetheless. Implications for optimizing cognitive test performance are discussed.