Microsatellite DNA markers for the ground beetle Pterostichus oblongopunctatus F.
Version of Record online: 6 FEB 2004
Molecular Ecology Notes
Volume 4, Issue 1, pages 113–115, March 2004
How to Cite
Lagisz, M. and Wolff, K. (2004), Microsatellite DNA markers for the ground beetle Pterostichus oblongopunctatus F. Molecular Ecology Notes, 4: 113–115. doi: 10.1111/j.1471-8286.2004.00586.x
- Issue online: 6 FEB 2004
- Version of Record online: 6 FEB 2004
- Received 5 June 2003; revision accepted 3 December 2003
Six microsatellite DNA loci were isolated from the ground beetle Pterostichus oblongopunctatus F. (Coleoptera, Carabidae) to study population structure. Each locus was polymorphic, with the number of alleles ranging from three to six. Observed heterozygosity varied between 0.20 and 0.67. All six loci were tested for amplification in the closely related P. quadrifoveolatus and they were shown to be polymorphic. The primers developed were used in multiplex polymerase chain reactions.