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Microsatellite DNA markers for the ground beetle Pterostichus oblongopunctatus F.

Authors

  • M. Lagisz,

    Corresponding author
    1. Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 3, 30-387, Krakow, Poland,
      Malgorzata Lagisz. Fax: 048 12 629 09 27; E-mail: lag@eko.uj.edu.pl
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  • K. Wolff

    1. School of Biology, University of Newcastle, Ridley Building, Claremont Road, Newcastle upon Tyne NE1 7RU, UK
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Malgorzata Lagisz. Fax: 048 12 629 09 27; E-mail: lag@eko.uj.edu.pl

Abstract

Six microsatellite DNA loci were isolated from the ground beetle Pterostichus oblongopunctatus F. (Coleoptera, Carabidae) to study population structure. Each locus was polymorphic, with the number of alleles ranging from three to six. Observed heterozygosity varied between 0.20 and 0.67. All six loci were tested for amplification in the closely related P. quadrifoveolatus and they were shown to be polymorphic. The primers developed were used in multiplex polymerase chain reactions.

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