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Characterization and PCR multiplexing of novel highly variable tetranucleotide Atlantic salmon (Salmo salar L.) microsatellites

Authors


E. Verspoor. Fax: + 44 (0)1796 473523; E-mail: E.Verspoor@marlab.ac.uk

Abstract

Seven novel and highly variable tetranucleotide microsatellite markers, and conditions for multiplexing and simultaneous genotyping six of these in a single run, are described for Atlantic salmon. These provide a highly informative and cost-effective set of molecular markers for genetic studies on cultured and wild populations of the species. The primers sets showed cross-species amplification of appropriately sized amplified products in a number of other salmonid species and suggests the primer sets may have wider application.

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