Present address: Arnold Arboretum of Harvard University, 22 Divinity Avenue, Cambridge, MA 02138, USA.
Universal primers for the amplification of chloroplast microsatellites in grasses (Poaceae)
Article first published online: 15 APR 2004
Molecular Ecology Notes
Volume 4, Issue 2, pages 262–264, June 2004
How to Cite
Provan, J., Biss, P. M., McMeel, D. and Mathews, S. (2004), Universal primers for the amplification of chloroplast microsatellites in grasses (Poaceae). Molecular Ecology Notes, 4: 262–264. doi: 10.1111/j.1471-8286.2004.00636.x
- Issue published online: 15 APR 2004
- Article first published online: 15 APR 2004
- Received 24 September 2003; revision accepted 4 February 2004
- simple sequence repeat;
- universal primers
Attempts to design truly universal primers to amplify chloroplast microsatellites have met with limited success due to nonconservation of repeat loci across widely divergent taxa. We have used the complete chloroplast genome sequences of rice, maize and wheat to design five pairs of primers that amplify homologous mononucleotide repeats across the Poaceae (grasses). Sequencing confirmed conservation of repeat motifs across subfamilies and a preliminary study in Anthoxanthum odoratum revealed polymorphism at two loci with a haplotype diversity value of 0.495. These primers provide a valuable tool to study cytoplasmic diversity in this extensively studied and economically important range of taxa.