PCR primers for fish G6PD gene intron and characterization of intron length variation in the albacore Thunnus alalunga


S. Chow. Fax: + 81-543-35-9642; E-mail: chow@affrc.go.jp


Polymerase chain reaction (PCR) primers to amplify the fourth intron of glucose-6-phosphate dehydrogenase (G6PD) gene were designed. A large length variation of amplified fragment was observed in the Atlantic albacore sample with a moderate level of heterozygosity (HE = 0.488). Nucleotide sequence analysis revealed deletion or insertion of a large nucleotide block (110 base pairs) to be responsible for the length difference. Successful amplification of single or two fragments was confirmed in the northern bluefin tuna and Pacific saury, indicating the wide cross-species applicability.