Novel microsatellites from the green swordtail (Xiphophorus hellerii) also display polymorphism in guppy (Poecilia reticulata)


Laszlo Orban. Reproductive Genomics Group, Temasek Lifesciences Laboratory, 1 Research Link, The NUS, Singapore 117604. Fax: 65-6872-7007; E-mail:


The green swordtail (Xiphophorus hellerii; Poeciliidae) is a popular ornamental freshwater fish species. Fourteen microsatellites were isolated from a green swordtail genomic library enriched for CA-repeats. Thirteen microsatellites were polymorphic in green swordtail; interestingly four of them were tetranucleotide-type. Cross-species amplification showed that 10 of the 14 microsatellites were polymorphic in guppy (Poecilia reticulata; Poeciliidae) as well. No significant directional difference of allele length was seen between the two species at any of the loci tested.