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Isolation of seven polymorphic microsatellite loci, using an enrichment protocol, in the high Andean Asteraceous Chaetanthera pusilla

Authors


I. Till-Bottraud. †Postal address: LECAUJF, BP 53, 38041 Grenoble cedex, France. Fax: + 33 4 76 51 42 79; E-mail: irene.till@ujf-grenoble.fr

Abstract

We report the development of seven microsatellite markers in the high Andean Asteraceae Chaetanthera pusilla. An enrichment protocol was used to isolate microsatellite loci, and polymorphism was explored with samples from two natural populations collected in the high Andes at La Parva and Valle Nevado (Chile). We found a high level of polymorphism, heterozygote deficiency and strong differentiation among populations. Four of the seven loci successfully cross-amplified in other Chaetanthera species.

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