Microsatellite markers for an endangered cyprinid species of the Mekong River, the seven-line barb (Probarbus jullieni Sauvage, 1880) were developed from the wild caught samples using (GT)15 probe. The number of alleles per locus ranged from seven to 16. The expected heterozygosities ranged from 0.47 to 0.91. Also, these primers were successfully amplified in the two closely related species, P. labeamajor and P. labeaminor. These markers have proven to be very useful for the population genetic structure study in this species and other related cyprinids.